Fabrication of Laminated Paper-based Analytical Devices (lpad) for Cotinine Detection
نویسندگان
چکیده
The majority of paper-based microfluidic devices were produced by patterning hydrophobic zones via photoresist or wax. Others were created by cutting paper using a laser. We present a fabrication method by craft-cutting and lamination, in a way similar to making an identification card. The method employs a digital craft cutter and roll laminator to produce laminated paper-based analytical devices (LPAD). Lamination with a plastic backing provides the mechanical strength for a paper device. The devices have been exploited for the detection of glucose in urine with colorimetric assays and for the measurement of cotinine in mouse serum using chemiluminescence immunoassay.
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